Distribution of sialoglycoconjugates in the rat cerebellum and its change with aging.

نویسندگان

  • Tasuku Sasaki
  • Yoshihiro Akimoto
  • Yuji Sato
  • Hayato Kawakami
  • Hiroshi Hirano
  • Tamao Endo
چکیده

We examined the distribution of sialoglycoconjugates in the cerebellum of 9-week-old and 30-month-old rats using light microscopy and electron microscopy in combination with two lectins, Maackia amurensis lectin (MAL) for Sia(alpha)2-3Gal and Sambucus sieboldiana agglutinin (SSA) for Sia(alpha)2-6Gal. Each lectin showed characteristic staining patterns. In young adult rats, MAL stained a strongly granular layer, a weakly molecular layer, and the medullary lamina, while SSA more strongly stained the medullary lamina than the molecular and granular layers. After aging, different staining patterns were obtained. Intense SSA reactivity was observed in the granular layer and intense MAL reactivity was observed in the medullary lamina of the aged groups. The reactivity of Purkinje cells with MAL was downregulated in the aged rats. These results indicated that Sia(alpha)2-3Gal and Sia(alpha)2-6Gal were expressed in distinct regions of the rat cerebellum and that their expression patterns changed in the aged brain.

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عنوان ژورنال:
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

دوره 50 9  شماره 

صفحات  -

تاریخ انتشار 2002